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SOP (Standard Operating Procedure) for FTIR Download Notes PDF BPharmacy 7th Semester 2022
π BP701T Instrumental Methods of Analysis
Title: |
SOP (Standard Operating Procedure) for FTIR |
Course: | BPharmacy |
Semester/Year: | 7th Semester |
Subject: | BP701T Instrumental Methods of Analysis |
Session: | 2022 |
Category: | Notes |
BPharmacy Practical and Experiments Standard Operating Procedure (SOP) Pharmaceutical Engineering |
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SWAMI DAYANAND POST GRADUATE INSTITUTE OF
PHARMACEUTICAL SCIENCES ROHTAK
SOP for FTIR (Fourier Transform Infrared Spectroscopy)
PROCEDURE FOR OPERATION AND CALIBRATION OF FTIR:
Start-up of FTIR Software
β Clean the Instrument and surroundings.
β Connect the instrument with the power supply.
β Switch on the instrument and Computer system.
β Double click on βIR Solutionβ Icon.
β After No. of internal operation.
β Select the βMeasureβ and select Measurement from the main menu, further select βinitializeβ
from the measurement tab.
β Press βYesβ then following screen will appear:
β Select File to load standard method for analysis or fill the desired parameter of the method.
β Click on file,
β Select the above option to enter the main window and select the standard method for analysis
or prepare new method as per STP.
β After selecting the standard method, double click on method or Open it from main window.
A dialog box will appear and ask about downloading parameter of Method.
β Press βOKβ to download the method parameters
β After completion of method downloading,
β Click on βBKGβ for background correction. A dialog box will appear.
β Press βOKβ if the Sample compartment is ready for Background.
β Select measure from the above screen window, fill all the required (sample/standards
information) in comment/Date file and select sample for sample analysis.
β For the Sample Analysis click on the Data path file and give the appropriate file path for
saving the data.
β Copy the file name and paste the data in the comment and click on the sample tab.
β After scanning the sample click on βCalculateβ and then βOKβ on the Peak Table.
β The number of peaks in the Peak Table must be between 15 to 25.
β To adjust the number of peaks, click on βCalculateβ and then increase or decrease the βMin
Areaβ and then click on βOKβ.
β Click on βWindowβ from the main menu bar and select βJoin Visibleβ.
β Then click on βManipulation 2β and select Purity.
Downloaded from HK Technical PGIMS (pgims.hktechnical.com)
β Right click on the sample and select send to source similarly right click on the reference and
select send to reference.
β Send to reference
β Click on βCalcβ, then βOKβ, select the print command from the main window.
β Click on βOKβ and select the desired report template for the Peak purity Graph as shown
below.
β Select the Report format and click on βOpenβ or double click on Report format. Press βOKβ
for printing the document.
β For Peak table: select the Manipulation 1 and select Peak table.
o After selecting the peak table,
o Click on βCalcβ then βOKβ.
β Select βPrintβ from the above screen.
β Select the report format as βpeak tableβ and print. As per the above-described procedure.
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