SOP (Standard Operating Procedure) for FTIR Download Notes PDF BPharmacy 7th Semester 2022

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SWAMI DAYANAND POST GRADUATE INSTITUTE OF
PHARMACEUTICAL SCIENCES ROHTAK
SOP for FTIR (Fourier Transform Infrared Spectroscopy)
PROCEDURE FOR OPERATION AND CALIBRATION OF FTIR:
Start-up of FTIR Software
● Clean the Instrument and surroundings.
● Connect the instrument with the power supply.
● Switch on the instrument and Computer system.
● Double click on “IR Solution” Icon.
● After No. of internal operation.
● Select the “Measure” and select Measurement from the main menu, further select “initialize”
from the measurement tab.
● Press “Yes” then following screen will appear:
● Select File to load standard method for analysis or fill the desired parameter of the method.
● Click on file,
● Select the above option to enter the main window and select the standard method for analysis
or prepare new method as per STP.
● After selecting the standard method, double click on method or Open it from main window.
A dialog box will appear and ask about downloading parameter of Method.
● Press “OK” to download the method parameters
● After completion of method downloading,
● Click on “BKG” for background correction. A dialog box will appear.
● Press “OK” if the Sample compartment is ready for Background.
● Select measure from the above screen window, fill all the required (sample/standards
information) in comment/Date file and select sample for sample analysis.
● For the Sample Analysis click on the Data path file and give the appropriate file path for
saving the data.
● Copy the file name and paste the data in the comment and click on the sample tab.
● After scanning the sample click on “Calculate” and then “OK” on the Peak Table.
● The number of peaks in the Peak Table must be between 15 to 25.
● To adjust the number of peaks, click on “Calculate” and then increase or decrease the “Min
Area” and then click on “OK”.
● Click on “Window” from the main menu bar and select “Join Visible”.
● Then click on “Manipulation 2” and select Purity.
Downloaded from HK Technical PGIMS (pgims.hktechnical.com)
● Right click on the sample and select send to source similarly right click on the reference and
select send to reference.
● Send to reference
● Click on “Calc”, then “OK”, select the print command from the main window.
● Click on “OK” and select the desired report template for the Peak purity Graph as shown
below.
● Select the Report format and click on “Open” or double click on Report format. Press ‘OK’
for printing the document.
● For Peak table: select the Manipulation 1 and select Peak table.
o After selecting the peak table,
o Click on “Calc” then “OK”.
● Select “Print” from the above screen.
● Select the report format as ‘peak table’ and print. As per the above-described procedure.

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